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msrA gene sequences were obtained from the National Center for Biotechnology Information (NCBI) database and primers were designed using the LAMP primer designing software PrimerExplorer v.4, which together recognize seven distinct regions of the msrA gene.
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Chen et al. used P. vivax primers to detect microscopically positive P. vivax clinical samples using the LAMP assay [13].
Two novel sets of loop-mediated isothermal amplification (LAMP) primers were designed to detect either representative freshwater (Aeromonas) or marine (Vibrio, Photobacterium, Listonella) bacterioplankton (aquatic bacteria) in one tube using the LAMP technique.
Be very careful when using the lamp.
The LAMP primers were designed using the PRIMEREXPLORER V4 software program (http://primerexplorer.jp).jp
The structure of the LAMP primers and their complementarity to target DNA used in this study are shown in Figure 3B.
The efficacy of LAMP using the new primer set was assessed in this study in comparison to that of a previously described set of LAMP primers as well as with microscopy and real-time PCR as reference methods for detecting P. falciparum.
The primer pairs used in LAMP are given specific designations; LAMP primers that generate hairpin loops, the outer displacement primers, and LOOP primers that accelerate the reaction by amplifying from the hairpin previously created by the LAMP primers [ 13, 14].
Use the lamps all on Ranged.
In contrast, only H. diffusa was detected in specific LAMP using the specific primers S_F3 and S_FIP.
In this study, we used LAMP primer sets designed from EMA-1 and Bc 48 genes for detection of Theileria equi and Babesia caballi infections, respectively.
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