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Alkaline phosphatase (ALP) was estimated using the kit supplied by Sentinel Diagnostics, Italy.
Blood urea nitrogen was measured using the kit supplied by Dr. Reddy's Laboratories (diagnostic division), Hyderabad, India, according to the manufacturer's instructions.
The Cy3 and Cy5 "tagged" cDNAs were combined and then hybridised to the array by overnight incubation in a humidity chamber at 65°C using the kit supplied non-formamide SDS-based buffer and a poly T based blocker, as per manufacturer's specifications.
The Cy3 and Cy5 "tagged" cDNAs were combined and then hybridized to the array by overnight incubation in a humidity chamber at 65°C using the kit supplied SDS-based buffer and a poly-T-based blocker, as per manufacturer's specifications.
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Serum low molecular weight adiponectin concentration was determined by ELISA method using the kits supplied by Glory Science Co., Ltd.
Prior to customizing the TaqMan Protein Expression Assay for SH2-PLA (i.e., modular domain binding assay), we tested the performance of the original proximity ligation assay using the kit-supplied anti-ICAM1 assay probes and Raji B-cell lymphoma lysate.
The isolation of total RNA from snap-frozen parotid gland tissues, in sample sizes ranging from 10 to 30 mg, was performed using the RNeasy kit supplied with the PARIS system (Ambion, Austin, TX, USA), according to the manufacturer's protocol.
SA-βgal expression was determined using the staining kit supplied by Cell Signaling Technology.
Serum aspartate aminotransferase (AST) activity was estimated using the modified kinetic method of Schumann and Klauke [ 16] using a kit supplied by Human, Germany, according to the instructions of the supplier.
Serum alkaline phosphatase (ALP) activity was estimated using the modified kinetic method of Tietz and Shuey [ 17] using a kit supplied by Human, Germany, according to the instructions of the supplier.
Serum alanine aminotransferase (ALT) activity was estimated using the modified kinetic method of Srivastava et al. [ 15] using a kit supplied by Human, Germany, according to the instructions of the supplier.
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