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Levels of Fe(II) and total Fe were analysed in cell lines using the kit from Abcam and following the manufacturer's instructions.
ZAM and Idefix mRNA expression was detected by in situ hybridization using DIG-labelled RNA probes transcribed from the pBS plasmids containing ZAM (3830 to 8040) or Idefix (4866 7191) fragments, using the kit from Roche.
ICS for FOXP3 was carried out using the kit from eBioscience according to manufacturer's instructions.
After 2 h at 37°C bound HRP was detected using the kit from Kirkegaard & Perry Laboratories.
Plasmid isolation was performed using the kit from Himedia and DNA purification was done using the Sure-Extract PCR Cleanup and Gel Extraction Kit from Genetix.
Plasma levels of 25-OH-VD were determined using an ELISA kit (Eagle Biosciences) and those of VDBP using the kit from ALPCO.
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aMT6s was assayed using the kits from IBL Immuno-Biological Laboratories, Hamburg, Germany.
The fecal TG and TC levels were determined using the kits from Nanjing Jiancheng Bioengineering Institute (Nanjing, China).
For CSA technique, we used the kit from DAKO according to the supplier's recommendations.
Then the RNA products were used to synthesize the first strand cDNA using the Kit (#K1622) from Fermentas Company.
RNA was isolated from cells using the RNeasy kit from Qiagen.
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