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Using the kinetic mode, assay time can be reduced substantially to 10 min for one measurement with the comparable sensitivity and single-base mismatch differentiating ability as were obtained by the maximum intensity detection mode.
Videos were recorded with manufacturer's software, using the kinetic mode with 50 100 ms exposure.
Each dose was measured in triplicate and compared with a blank for about 15 min using the kinetic mode.
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This choice allows one to compute the potential energy of the plate using the kinetic energy of single modes of free-edge plates.
GGT was measured using the kinetic colorimetric method [ 29].
Serum creatinine concentration was measured using the kinetic Jaffe assay.
All the measurements were taken using the spectrophotometer in the kinetic mode of 4 minutes with 1-minute intervals.
To determine turnover rates of individual substrates a Spectra MAX Gemini EM fluorimeter (Molecular Devices) operating in the kinetic mode in 96-well plates was used.
Notably, at some potentials the deactivation was not only slowed but the kinetic mode was completely altered.
Light scattering at 400 nm was monitored for 1 h in the kinetic mode.
Appropriate laccase dilutions were prepared, such that 20 μL aliquots produced a linear response in the kinetic mode.
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