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Using the kinetic analyses of UDP-Glc-dependent MrSGT, wereere able to confirm the promiscuity of recombinant MrSGT, in contrast to the previously discovered bacterial SGTs.
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The peak areas determined from the nonlinear least-squares fits were used for the kinetic analyses.
Further explanation of the simplifications and constraints used in the kinetic analyses is found in the Supporting Information.
To obtain kinetic information we can analyse the data using the kinetic scheme developed for the fibrillation mechanism of human calcitonin by Kamihira et al.[ 9] and subsequently modified for β-amyloid fibrillogenesis by Sabaté et al.[ 10] and Iqbal et al.[ 6] for HbS, which was fitted to our data.
Extracts from polycarbonate filters were analysed in duplicate for β-glucan from fungi and pollen using the kinetic, chromatic Fungitic G Test (Seikaga Co., Tokyo, Japan).
GGT was measured using the kinetic colorimetric method [ 29].
Serum creatinine concentration was measured using the kinetic Jaffe assay.
In the kinetic analyses using Lineweaver-Burk plofs of 1/velocity and 1/substrate concentration, hirsutrin showed competitive inhibition against rhAR.
For the kinetic analyses, the production rates of sterol glucosides were plotted vs. the respective substrate concentrations using the SigmaPlot enzyme kinetic module (Systat Software Inc., ver. 12.0, San Jose, CA, USA).
Cells showing mixed currents due to non-stoichiometric association were not included in the kinetic analyses.
As observable we use the kinetic energy.
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