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Replication genotyping of seven SNPs in KORA S4 samples (n = 1,830) was performed using the iPLEX assay (Sequenom).
One nanogram of DNA was assayed using the iPLEX assay on the MassARRAY MALDI-TOF mass spectrometer (SEQUENOM, Hamburg, Germany).
The candidate gene single nucleotide polymorphism (SNP) analyses were performed using the iPLEX assay in conjunction with the Sequenom MassARRAY platform.
Stage 2 genotyping (KORA S4 cohort, n = 1830) of 74 SNPs was performed using the iPLEX assay (Sequenom; primers are given in Supplementary Material, Table S5).
Replication sample I was genotyped using the iPLEX assay on the MassARRAY MALDI‐TOF mass spectrometer as described (Oeth et al, 2009).
Paired cDNA and genomic DNA samples, in triplicate, were processed using the Iplex assay and detected by Sequenom massI-TOF maspectrometrytry.
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The majority of SNPs were genotyped at the Broad Institute for Genotyping and Analysis (http://www.broad.mit.edu/gen_analysis/genotyping/) with Sequenom's MassARRAY platform (San Diego, CA, USA) using the iPlex SNP assay design system [4].
A number of genes did not consistently show monoallelic expression using the iPLEX Gold assay.
Genotyping was performed using the iPLEX genotyping assay (Sequenom Inc, San Diego, CA, USA) at the Centre for Integrative Genetics CIGENEE) genotyping core facility at the University for Environment and Biosciences, Ås, Norway.
Multiplex genotyping of genomic DNA and cDNA was performed by using the iPLEX Gold assay on the MassARRAY platform (Sequenom) at the Génome Québec Innovation Centre (Montréal, PQ, Canada).
(4) Only SNP that met all requirements were multiplexed for genotyping the F1 parents and their F2 progeny using the iPlex Gold assays on the MassARRAY platform (Sequenom, San Diego, CA) according to the manufacturer's instructions at the Genome Québec Innovation Center (McGill University, Montréal, QC, Canada).
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