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Bands were quantitated using the integrated density function of ImageJ.
Images were analyzed with the NIH ImageJ software (v. 1.43) http://rsbweb.nih.gov/ij/ using the integrated density function.
Labelling intensities of total, nuclear and cytoplasmatic Cyclin D1 were obtained in each image using the integrated density option of the Image-J software.
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A standard curve was plotted using the integrated densities of known mSEB standards.
The protein expression levels were analyzed using a semiquantitative method of relative optical density that was calculated using the integrated optical density of the target band divided by the integrated optical density of the reference band.
The staining intensity and extent were scored by two independent pathologists who were blind to the clinical outcomes using the integrated optical density (IOD) calculated by Image‐Pro Plus version 6.0 software (Media Cybernetics Inc., Bethesda, MD, USA).
He welcomes inquiries into using the integrated capital model.
LDL uptake was measured using the ImageJ integrated density measurement tool.
MCID software was used to analyze the integrated density of labeled axonal fibers and synaptophysin in the IBZ.
The pixel density was determined after background subtraction and used to calculate the integrated density of a selected band.
Density of ht2cr mRNA and Pomc mRNA was examined in adjacent sections of brain tissue using densitometry analysis by computing the integrated density (the sum of the gray values minus background) using ImageJ (National Institutes of Health).
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