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Multiple linear regression analysis using the insulin levels as dependent variable showed a significant association for MVC (P=0.047), weight (P=0.017), and waist circumference (P=0.000).
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Plasma insulin levels were measured using the Insulin Ultrasensitive (Mouse) EIA method (Alpco, Salem, NH).
Insulin levels were measured using the Insulin Enzyme Immunoassay Kit (Cayman Chemical, Cat. #589501, Ann Arbor, MI 48108, USA) according to the manufacturer's instructions as previously described [ 29].
Insulin levels were measured using the Insulin Enzyme Immunoassay Kit (Cayman Chemical, Cat. 589501, Ann Arbor, MI 48108) according to the manufacturer's instructions as previously described [ 18].
Because of the different assays used, quantitative comparisons among the insulin levels after insulin lispro and the human insulin formulations are not useful.
Many of the formulae for indices derived from fasting specimens are simple using the fasting insulin level alone.
The insulin levels were measured using a commercial rat insulin ELISA kit (Mercodia, Sweden).
The insulin levels were measured using a commercial rat insulin ELISA kit (cat. no. 10-1113-01, Mercodia, Sweden).
The homeostatic model assessment of insulin resistance (HOMA-IR) was calculated using fasting insulin levels (mU/l) and simultaneous fasting serum glucose (mmol/l) using the following equation: HOMA-IR = (fasting insulin × fasting glucose)/22·5.
First- and second-phase insulin secretions were estimated using the glucose and insulin levels at 0 and 120 min during the OGTT and BMI measurements [16].
Clamp-measured insulin sensitivity provides an accurate estimate of whole-body insulin sensitivity, which is largely determined by skeletal muscle because the used insulin levels almost fully suppress endogenous glucose production (18).
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