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Data was analysed using the instrument software provided by the manufacturer.
Spectra were processed using the instrument software (TopSpin, Bruker Biospin, Inc ., and analysis was conducted using iNMR (Nucleomatica, Molfetta, Italy; http://www.inmr.net), a computer program designed to aid in resonance assignments.
Data were analyzed using the instrument software.
The acquisition of MS/MS spectra was performed with an automated data-directed switching between the MS and MS/MS modes using the instrument software (MassLynx V4.0 SP4).
Second derivative spectra were calculated using the instrument software and 13 data points.
Peak parameters (peak area, height, width, as well as peak spectrum) were obtained using the instrument software.
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The Ct values were determined using the instrument's software.
Transcript abundance was calculated using automatic baseline and threshold settings using the instrument's software.
In a typical experiment, 16 injections of GTPase into the cell were recorded at 15°C, and relevant thermodynamic parameters were analyzed and calculated using the instrument's software (Origin).
HS images were analyzed using the instrument's ENVI software (ITT Visual Information Solutions, Boulder, CO, USA).
Chromatography was performed online using the instrument's control software MassLinx of Q-TOF Ultima™ global.
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