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PCR products were cloned into pENTR/D-TOPO vector (Invitrogen) using the instructions provided by the manufacturer.
You can obviously build one of these yourself using the instructions provided, but it sure would feel special to pull a machine out of a box that brought so many men to ruin and created the foundations for modern computing.
The area under this peak was calculated using the instructions provided on the Image J website.
The disks were then removed and stained with Live/Dead BacLight stain (Invitrogen), using the instructions provided by the manufacturer.
Like Daniel, I also downloaded and built the software using the instructions provided in the ReadTheDocs documentation.
The data can either be entered into a spreadsheet, using the instructions provided, or by using the specifically developed Microsoft Access form-based entry tool.
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Other types of kits exist as well, so be sure to use the instructions provided with your kit.
Biotinylated anti-mouse antibody was used as secondary antibody and streptavidin horseradish peroxidase (Zymed laboratories, San Francisco, CA) methods were used following the instructions provided by the manufacturer.
Real-time quantitative polymerase chain reaction (PCR) was performed on an ABI Prism 5700 sequence detection system (Applied Biosystems, Foster City, CA, USA) using SYBR Green Applied Biosystemss) following the instructions provided by the manufacturer.
Biotinylated anti-mouse antibody was used as a secondary antibody and streptavidin horseradish peroxidase (Zymed laboratories, San Francisco, CA) methods were used according to the instructions provided by the manufacturer.
For real-time quantitative PCR analysis, the QuantiTect SYBR Green PCR kit (Qiagen, USA) was used according to the instructions provided by the vender.
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