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After hybridization, further steps were performed using the instruction of v2.0 miRCURY LNA microRNA ISH Optimization Kit for FFPE (Exiqon).
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Likewise, if you are writing a research paper, start to make your outline using the instructions of the journal for the format/headings the journal requires; e.g., Summary, Introduction/Background, Methods, Discussion, References.
Cutoffs were set by using the instructions of the manufacturer.
The experiment was carried out using the operation instruction of manufacturer (GE healthcare company).
Also, the literature provides few examples for accessible laboratory equipment used in the instruction of STEM courses at the postsecondary level.
The observatory would be used for the instruction of students into the early twentieth century.
For detection and quantification of apoptosis, the TUNEL commercial kit for cell death detection (Roche) was used following the instructions of the manufacturer.
Since July 2007, an automated commercial GuSCN-based method was used, according the instructions of the manufacturer (Easy MAG 2.0, bioMérieux, Marcy l'Étoile, France).
Antibodies for CD31 (Santa Cruz, SC-1506), α-SMA (Abcam, ab-5694), and VEGF (Santa Cruz, SC-152) were obtained commercially and used following the instructions of the providers.
The WST-1 kit was used according to the instruction of the provider.
Total RNA was isolated using Trizol reagent following the instruction of the supplier (Gibco-BRL).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com