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Using the innate fluorescence properties of ADPM06, its biodistribution pattern in major organs after parenteral delivery has been established.
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This latter property provides straightforward evidence of the innate fluorescence of the trapped macrocycles.
In such a case, the innate fluorescence property allows QDs to serve as a single vehicle for drug/biomolecule delivery, visualization and monitoring.
The percentages of GFP+ cells within the total WBC population and subsets (lymphoid and myeloid) were determined on the basis of the innate fluorescence of GFP.
Mitochondrial ROS was detected using the fluorescence indicator MitoSOX (Life Technologies Inc.).
Samples were visualized using the fluorescence microscope Leica TCS SP5 (Leica Microsystems, Wetzlar, DEU).
Determine Sav FBS using the B4F absorption-fluorescence assay.
(E) Immuno-fluorescence showing DNA damage in HeLa cells using the γH2Ax marker (red fluorescence).
FLIP rates were calculated from fluorescence intensity measurements using the Zeiss LSM software, setting the initial fluorescence to 100% signal.
Fluorescence was measured using the plate reader at 560/590 nm.
AF647 fluorescence was measured using the same settings used for Cy5 and described above.
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