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Additionally, we successfully used the on-chip activity assay using the indirect probe to determine TG2 and FXIII activities in Hela cell lysates and human plasma samples, respectively.
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After Cy3 and Cy5 labelling of the probes using the indirect labelling method, individual NN and MM samples were hybridised in eight independent dye-swap experiments.
The stiffness modulus and indirect tensile strength of the specimens were measured using the indirect tensile tests.
The immunohistochemistry was carried out using the indirect immunoperoxidase method.
The analysis will be calculated using the indirect standardisation method.
Secondly, I use the indirect method.
Use the indirect method.
You can also use the indirect heat method of cooking.
15 μg of total RNA were converted in either Cy3- or Cy5-labeled cDNA probe using the Superscript indirect cDNA labelling kit (Invitrogen Life technologies, Irvine, CA).
Aminoallyl-cDNA was synthesised from 15 μg of total RNA using the SuperScript indirect cDNA labelling system (Invitrogen Corporation, CA, USA) and labelled using ARES Alexa Fluor labelling kits (Molecular Probes, OR, USA).
Labeled probe for hybridizations with the NSF45K microarray were prepared from 1 2 µg mRNA samples using the SuperScript™ indirect cDNA Labeling System (Invitrogen, Carlsbad, CA).
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