Sentence examples for using the indicated primary from inspiring English sources
After SDS PAGE and transfer of proteins onto nitrocellulose membranes (GE Healthcare), specific proteins were detected using the indicated primary antibodies and horseradish peroxidase-conjugated donkey anti-rabbit and sheep anti-mouse antibodies (GE Healthcare).
Proteins were detected using the indicated primary antibodies and the Odyssey infrared imaging system (Odyssey).
Immunoblotting was performed using the indicated primary antibodies and revealed using the ECL+ Detection Kit (GE Healthcare).
Proteins present in the extracts were resolved by LDS-PAGE using 4 12% BisTris gels (NuPAGE; Invitrogen), transferred to nitrocellulose membranes, and probed for tagged protein by immunoblotting using the indicated primary antibody.
Immunoblotting was performed using the indicated primary antibodies and analyzed using secondary antibodies for enhanced chemiluminescence.
The immune complexes were subjected to Western blot analysis as described above using the indicated primary antibodies.
A time-varying filter is designed using the indicated local maxima comprised in the score matrix.
(B) Immunohistochemical analysis using the indicated antibodies in adjacent non-tumor tissues.
(D ) The occupancy of histone H3 was measured by ChIP using the indicated amplicon in the indicated strains.
Right panel, the occupancy of S2P was measured by ChIP using the indicated amplicons in the indicated strains.
Immunoreactive proteins were visualized with the indicated primary antibodies using enhanced chemiluminescence (ECL) (Amersham) followed by densitometry.
