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The proteins were detected by immunoblotting using the indicated antibodies.
The lysates and immunoprecipitates (IP) were analyzed by immunoblot using the indicated antibodies.
NEDP1 and NEDD8 were detected by Western blotting using the indicated antibodies.
The resulting samples and lysates were analyzed by immunoblotting using the indicated antibodies.
The endogenous ubiquitin and SUMO2/3 conjugations of global proteins were determined by IB using the indicated antibodies.
Then, isolated protein samples were subjected to 10% SDS-PAGE separation and subsequent Western blot analysis using the indicated antibodies.
Immunoblot assays were performed using the indicated antibodies.
Immunoblotting were performed as previously described using the indicated antibodies [50], [51].
If the screen is open ended, progress can be followed using the indicated equations.
Voltage ramp- or step-induced currents were acquired using the indicated protocols.
The eluted proteins were resolved on 10% SDS-PAGE gel electrophoresis for western blotting analysis using the indicated antibodies.
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