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PTEN was scored semiquantitatively using the immunoreactive score (IRS).
Intensities of the immunoreactive bands were measured using the ImageJ software.
Blots were developed using SuperSignal reagents (Pierce Biotechnology), exposed to X-ray film and immunoreactive bands were quantified using the ImageJ software.
Quantification of immunoreactive signal was performed using the analysis software Quantity One (Bio-Rad) on a GS-800 Calibrated Densitometer (BIO-RAD).
Western blot data acquisition and analysis were accomplished by measuring the pixel density and area of immunoreactive bands from the ECL films using the ImageJ software (NIH).
Immunoreactivity was detected with an enhanced chemiluminescence method (ECL™ plus detection reagent, GE Healthcare) and immunoreactive bands were quantified by densitometry using the ImageJ software.
Immunoreactivity was detected with an enhanced chemiluminescence method (ECL plus detection reagent, GE Healthcare) and immunoreactive bands were quantified by densitometry using the ImageJ software in three independent experiments.
For quantification of western blot bands, blots were scanned and the signal intensity of the immunoreactive bands was quantified as previously described (Richter and Conti, 2004) using the ScionImage software program (Frederick, MD).
Intensities of immunoreactive bands were measured using the Quantity One software and normalized to β-actin levels.
Blots were scanned to obtain images and the immunoreactive bands were analyzed by densitometry, using the Quantity One software (BioRad).
Immunoreactive bands were quantified by using the ImageQuant TL software (GE Healthcare).
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