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Hippocampal mossy fiber boutons were reconstructed from image stacks using the Imaris surface tool (Bitplane, Zurich, Switzerland).
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Z stacks of confocal images of longitudinal sections from an injured sciatic nerve of PLP-EGFP mice stained for CD31 (blue) and neurofilament (NF, red) were processed using the Imaris software to make a 3D surface-rendered reconstruction of the interaction between the vasculature and Schwann cells.
The PCNA-positive beta-cells were counted manually, while the counting of the EGFP-positive and RFP-positive cells were performed using the Imaris Imaging Software (Bitplane).
Colocalization analyses were performed using the Imaris colocalization module (Bitplane AG, Zurich, Switzerland).
Image data obtained were processed by using the IMARIS software package (Bitplane AG, Zürich, Switzerland).
Confocal images were processed using the Imaris program (Bitplane AG, Zürich, Switzerland) and Photoshop 6.0 (Adobe System Inc).
Processes (filaments) and synaptic puncta within each section were traced in 3D using the Imaris 6.2 software (Bitplane).
The movie was processed using the Imaris software.
Three-dimentional imaging and cell quantification were realized using the Imaris 6.4.2 software (Bitplane AG, Zurich, Switzerland).
Cell and network volumes were created using the Imaris (Bitplane, Zurich, Switzerland) software package.
Mitochondrial PXN content (calculated as GFP-PXN intensity at/in the fit mitochondrial surface; not total cell GFP-PXN content), mean MitoTracker intensity, mitochondrial surfaces (structures)/cell (count of fitted mitochondria in the volume of the cell), and mean mitochondrial volume/cell were calculated using the Imaris software.
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