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Image data obtained were processed by using the IMARIS software package (Bitplane AG, Zürich, Switzerland).
Z-sections were collected and reconstructed into 3D images using the IMARIS software (Bitplane, Inc., Saint Paul, MN).
Images were acquired using Laser Sharp 2000 software then imported and further analyzed for detailed quantitative 3D analysis using the IMARIS software package (BitPlane Inc., MN, USA).
Images of each experiment were processed and analyzed as previously described [58] and visualized using the IMARIS software package (version 5.7.2, Bitplane).
The movie was processed using the Imaris software.
The total area of the VZ per section was calculated using the IMARIS software (Bitplane).
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We used the Imaris software to create 3D reconstructions of Drosophila muscle mitochondria from z-stack images.
Processes (filaments) and synaptic puncta within each section were traced in 3D using the Imaris 6.2 software (Bitplane).
Three-dimentional imaging and cell quantification were realized using the Imaris 6.4.2 software (Bitplane AG, Zurich, Switzerland).
The 3-D reconstruction of the FM1-43FX events was performed using the Imaris 6.1.5 software (Bitplane Scientific Solutions, Zurich, Switzerland).
A × 40/1.3 NA (numerical aperture) oil-immersion objective lens was used with a zoom factor of 4. Images were analysed using the Imaris analysing software (Bitplane).
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