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Confocal images were processed using the Imaris program (Bitplane AG, Zürich, Switzerland) and Photoshop 6.0 (Adobe System Inc).
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Colocalization analyses were performed using the Imaris colocalization module (Bitplane AG, Zurich, Switzerland).
Image data obtained were processed by using the IMARIS software package (Bitplane AG, Zürich, Switzerland).
Processes (filaments) and synaptic puncta within each section were traced in 3D using the Imaris 6.2 software (Bitplane).
The movie was processed using the Imaris software.
Hippocampal mossy fiber boutons were reconstructed from image stacks using the Imaris surface tool (Bitplane, Zurich, Switzerland).
Three-dimentional imaging and cell quantification were realized using the Imaris 6.4.2 software (Bitplane AG, Zurich, Switzerland).
Cell and network volumes were created using the Imaris (Bitplane, Zurich, Switzerland) software package.
The 3-D reconstruction of the FM1-43FX events was performed using the Imaris 6.1.5 software (Bitplane Scientific Solutions, Zurich, Switzerland).
For colocalization analyses, confocal images were analyzed using the Imaris 'Co-localization Module' and the Volocity 3D opacity utility.
Colocalization analysis was then performed on the deconvolved confocal stacks in 3D using the Imaris Coloc module (BitPlane AG).
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