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High resolution, three-dimensional (3-D) reconstruction of GFP positive cancer cells in PCLS was performed using the IMARIS image analysis software.
We also demonstrated that single-cell behavior on the slices can be observed by confocal microscopy using the IMARIS image analysis program.
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Hippocampal mossy fiber boutons were reconstructed from image stacks using the Imaris surface tool (Bitplane, Zurich, Switzerland).
Confocal images were processed using the Imaris program (Bitplane AG, Zürich, Switzerland) and Photoshop 6.0 (Adobe System Inc).
For colocalization analyses, confocal images were analyzed using the Imaris 'Co-localization Module' and the Volocity 3D opacity utility.
A × 40/1.3 NA (numerical aperture) oil-immersion objective lens was used with a zoom factor of 4. Images were analysed using the Imaris analysing software (Bitplane).
Image data obtained were processed by using the IMARIS software package (Bitplane AG, Zürich, Switzerland).
Confocal images of 18 biofilms were captured (ZEISS LSM780 Confocal System) and analyzed using the Imaris software package (Bitplane).
The 4D images produced by the time-lapse acquisitions were processed, clipped, examined and interpreted using the Imaris software.
Colocalization analyses were performed using the Imaris colocalization module (Bitplane AG, Zurich, Switzerland).
The movie was processed using the Imaris software.
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