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The morphology of algae was documented with the attached Olympus ColorView III and II cameras (Soft Imaging System GmbH, Münster, Germany) using the imaging software Cell^D and analySIS (Soft Imaging System GmbH).
Slides were imaged on an Olympus/3I Spinning Disk Confocal/TIRF Inverted Microscope and 6 8 random fields per coverslip were captured using the imaging software, SlideBook 5.0 (Intelligent Imaging Innovations Inc).
The images were acquired using the imaging software Metamorph 7, then processed in Adobe Photoshop 7.0.1 with adjustments of brightness and contrast.
The quantitative analysis image was performed using the imaging software Image J. The relative distribution of p27kip1 protein between the two compartments nucleo/cytoplasm, was calculated by measuring the pixel average signal both in the nucleus, cytoplasm and nucleo/cytoplasm compartments.
Superposition of the CFP and YFP images was carried out using the imaging software.
From the images, the average pore diameter was calculated using the imaging software ImageJ 1.46r using the multi-measure plugin by randomly considering 20 pores.
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All images were processed using the imaging processing software (Vitrea version 3.9.0.1, Vital images, Minnesota, U.S.A).
After 5 days in culture, the number of neurospheres generated per 10 cells was counted, and neurosphere diameter was measured using the imaging analysis software, ImageJ developed by U. S. National Institutes of Health, Bethesda, Maryland, USA (http://imagej.nih.gov/ij/, 1997 2012).
The pixel intensity of proSP-C or aquaporin 5 immunofluorescence (Figure 6A) was quantified in 5 random fields from each of 3 independent sections using the SlideBook imaging software (Intelligent Imaging Innovations).
Cerebellar herniation length was assessed from mid-sagittal T2 weighted images using the same imaging software, with a previously described technique [ 9].
The nuclei from apoptosis 'hotspot' areas were digitally counted (object counting) using the SimplePCI imaging software on random images (at least 10 images per tumor from multiple tumors per group) captured at ×250.
More suggestions(15)
using the visualization software
using the instrument software
using the imaging mode
using the imaging method
using the imaging processing
using the imaging system
using the imaging ability
using the imaging coil
using the imaging protocol
using the imaging plate
using the CELLQuest software
using the imaging sequence
using the imaging technique
using the imaging sensor
using the imaging analysis
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