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An initial pattern consisting of the matrices of the irradiated dots at constant intervals is prepared by raster scanning mode and it is developed by irradiation using the imaging mode in FIB at room temperature.
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The direct electron beam intensity used for the imaging mode was detected by a CCD camera.
Prior to in vivo imaging, the phantom was imaged using B-mode US and T2-weighted MRI, using the imaging parameters described above.
We have seen not only a change of direction of the PFR filaments but also that these filaments have higher tensile strength during flagellar bending as measured using the phase imaging mode, therefore agreeing with the theory proposed by [26].
The imaging mode permits use of high numerical aperture optics, allowing wide-field imaging from densely packed arrays.
In Fig. 2d the imaging mode based on the detection of secondary electrons is used.
Detailed images were obtained from small areas (100 μm × 100 μm with 256 × 256 pixel definition) using the high spatial imaging mode.
Ultrasound settings were adjusted to optimally demonstrate the lesion and anticipated needle tract, mostly using the tissue harmonic imaging mode.
Contrarily, the authors in[20 22] use the common linear frequency modulated (LFM) pulse sequence for CS-based SAR imaging using the spotlight mode.
The position and orientation of the zebrafish within the block was determined using secondary electron imaging mode (SEI, Everhardt Thornely Detector), whereas atomic number contrast of osmium within the tissue was imaged using a solid-state backscattered electron (BSE) detector [5], [3].
One solution is to use electron tomography, a technique for imaging three-dimensional structures, pioneered originally in the life sciences, but that has recently been modified, using alternative imaging modes, for the analysis of inorganic materials.
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