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After 5 days in culture, the number of neurospheres generated per 10 cells was counted, and neurosphere diameter was measured using the imaging analysis software, ImageJ developed by U. S. National Institutes of Health, Bethesda, Maryland, USA (http://imagej.nih.gov/ij/, 1997 2012).
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Prior to mechanical testing the integrated interface area of each sample was determined by optical microscopy using the imaging software analySIS 3.1 (SZX12, Olympus, Hamburg, Germany).
The morphology of algae was documented with the attached Olympus ColorView III and II cameras (Soft Imaging System GmbH, Münster, Germany) using the imaging software Cell^D and analySIS (Soft Imaging System GmbH).
The percentage of the viable and dead cells was analyzed using the cell imaging analysis software.
Quantification of cell cycle and cell death was performed using the BioQuant imaging analysis software.
Cortical reconstruction and subcortical volumetric segmentation were performed using the FreeSurfer imaging analysis suite (v5.1.0, http://surfer.nmr.mgh.harvard.edu/) [ 39].
Photographs were taken using a fluorescence macroscope (Carl Zeiss, Thornwood, NY, USA), and the number of macrophages that adhered to the mouse aorta were counted using the MetaMorph imaging analysis system (Molecular Devices) and normalized to the total aortic area.
A Leica DMR fluorescence microscope (DM4500B; Leica Microsystems) equipped with a high-resolution camera (Hamamatsu) and OpenLab software (Improvision) was used for all of the imaging analysis.
The imaging analysis was used to determine if both CD44 and liposomes were present on the target cells but absent on the control cells.
For the ROI analysis we used the imaging data of the GLD task and we looked at the contrast between homographs and controls.
The focus of the present paper is on using digital imaging analysis to evaluate the role of intra-raw material variability in use-wear formation by experimentally making and using five flake scrapers made from San Juan Fossiliferous chert (SJF) and five made from Yellow Silicified Wood (YSW).
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