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The synaptic punctae were detected, using the image analyzing system CellP (Soft Imaging System, Münster, Germany).
The number of penetrated neuroblastoma cells were then quantified using the image analyzing system ARGUS 20 (Hamamatsu, Hersching, Germany).
Size distribution measurements for Au particles were performed on digital images by using the image analyzing software Image-Pro.
For each mandible, the coordinates of 64 points at equal curvilinear distance along the outline were extracted based on photographs using the image analyzing software Optimas v. 6.5.
The percentage of TUNEL-positive cells per total number of cells was counted from the number of DAPI-stained nuclei in six different fields of each slide for each group using the image analyzing software.
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To determine the particle size and size distribution, the AFM images were analyzed using the image analyzer software.
To quantitate tumor suppressive effects, the total area of tumors in each section stained with H&E was calculated using the image-analyzing software Winroof (Mitani Corporation, Tokyo, Japan).
Gels were stained with Coomassie Blue and the final image analyzed using the Image Master Platinum software (Amersham).
Images were analyzed using the Image J software.
Root curvature angles were measured and analyzed using the Image J software (http://rsb.info.nih.gov/ij/).
Digital images were analyzed using the Image J software (version 1.42q, National Institute of Health, Bethesda, MD, USA) and measured for shape descriptors (area, perimeter and circularity).
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