Additional cases were annotated by single analysts using the image analysis software integrated in the OCT imaging system C7-XR.
The following mechanism is applied using the image analysis method to determine grain-size distribution: selecting the sampling site, imaging, and image analysis.
The length of profiles was measured using the image analysis software.
Spheroid radius was then measured from digital photomicrographs using the image analysis software ImageJ64-1.45i (http://imagej.nih.gov), calculating the spheroid projected areas and assuming a spherical shape.
The lateral size distributions of ~500 isolated GO sheets were determined from several SEM images and analysed using the image analysis software (ImageJ, http://rsb.info.nih.gov/ij/).
Before tracing the ROI, functional scans were aligned to a reference anatomical scan using the image analysis software ImageJ Wayne Rasbandd, http://rsb.info.nih.gov/ij/; Karl Schmidt http://www.quickvol.com).
The distance between two neighbouring collagen fibres was measured manually from the fluorescence microscopy images using the image analysis software, FIJI (Supplementary Fig. 4a).
Regions of interest [ROIs] were drawn using the image analysis software IRW from Siemens.
The number of the cells was determined using the image analysis software NIS-Elements (Melville, NY, USA).
The axonal lengths of neurons grown on different substrates were measured using the image analysis software as described.
Information on these properties were obtained from scanning electron microscopy (SEM) images using the image analysis tool ImageJ (NIH, Bethesda, MD, USA) [34].
