For that purpose, we also trained a SHMM that specifically models tail tail orientations using the identical initial settings.
To correct for bleed-through, images of HEK293 cells expressing GCaMP6s (in pcDNA3.1) were acquired upon stimulation with 2 μM ionomycin in varying external CaCl2 concentrations, using the identical acquisition settings as for the acquisition of the calibration curve.
For comparative analysis between the fluorescence signal of NCAM1 sorted cells, images were acquired using identical settings (exposure and gain setting) using Zeiss AxioVision Software Package and analysed using ImageJ software.
employed an automated 5 9 MHz transvaginal three-dimensional transducer (Voluson 730 expert, GE Medical System, France) to acquire power Doppler information from the uterus using identical settings and technique in all cases as follows: tissue harmonic imaging, mild; pulse repetition frequency, 0.6 khz; wall motion filter, low; gain, −7.8; high quality, rise 0.2.
Representative images of normal breast and breast cancers from the same sections were taken using identical settings at 20x magnification using a Leica DMI4000b inverted bright-field/fluorescence microscope.
In each experiment, images were acquired using identical settings and the same threshold was used for all groups.
To exclude artifacts resulting from small differences in buffers and incubation times, all samples were processed simultaneously, and images were recorded using identical settings on the microscope and the software.
First, using identical settings for the display, a metanet showed that, for the same 206 specimens, CO1 sequences present more divergence than psbA sequences, which means that CO1 had a higher rate of evolution than psbA in these organisms.
Images were acquired typically from 10 15 cells of similar size from each experimental condition using identical settings of the instrument.
