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In theory, the same approach of using the identical sequencing platform for the background strains could be used for simplex whole-exome analysis.
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SDs was identified using the identical whole-genome sequence comparison method [ 58].
The MAT loci of additional G. clavigera isolates from different geographic locations were also sequenced using the identical long-range PCR approach to investigate whether the truncated MAT1-1-1 gene could be unique solely to isolate SL-KW1407 (Table 1).
Instead, the SSA pathway only requires a single DNA duplex, and uses the repeat sequences as the identical sequences that homologous recombination needs for repair.
Ligation of universal adapters to DNA fragments creates products that are extended by PCR to produce barcoded samples containing the identical sequences used for Illumina TruSeq multiplexing (see Additional file 1; also TruSeq DNA Sample Preparation Guide, Part No. 15005180 Rev. A).
We compared our sequencing results using the same primers and confirmed that MLMTHR and MLM651 produced the identical sequences to those by using primers GMM.
Purified PCRb products were then sequenced with primers BFBX and BFX8 using identical sequencing conditions as for the mtDNA cytb sequencing.
Additional analyses were carried out separately for male and female samples using an identical sequence of models (with the exclusion of gender as a background characteristic).
In spite of this, the utility of using identical sequences enabled us to notice 26 inconsistent cases in the Ensembl database v50 human genome annotation.
To avoid undesired DNA rearrangement caused by using identical sequences (such as using same promoters for multiple gene expression in a single construct), we chose to use the maize ubiquitin promoter (Pubi, [ 24]) to drive the expression of α and β subunits of rP4H.
To check the significance of these scores, we randomly generated blocks with an identical size using the same sequences.
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