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In a separate study using the identical cell line and implantation protocol, SCID mice bearing orthotopic breast tumors were treated for a shortened period (7 days) with neoadjuvant sunitinib at a higher dose (120 mg/kg).
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In earlier studies using the identical cell-biological assay, similar effects on tryptophan metabolism have been found by investigating Hypericum perforatum extracts as well as Δ9-tetrahydrocannabinol and cannabidiol, indicating that the suppression of tryptophan breakdown and neopterin production might be an important but a more general aspect in the action of psychoactive compounds [ 36, 37].
Importantly, we used the identical cell proliferation assays for assessing GI in both the breast and the ovarian cancer cell line panels.
Thus, tumors were again induced by intracranial injection of T98G cells using the identical number of cells as in Figure 8 A,B.
Using the identical protocol of B16-F10 cell inoculation as above, we again found no significant differences between the two experimental groups after 17 days in tumor volume, edema formation and infiltrating immune cells.
We subsequently searched for subchromosomal regions that were clustered for efficient and inefficient replication loci in cds1Δ cells using the identical approach in wild type.
The identical cell samples used for proteomics analysis were used for RT-qPCR analysis.
This pooled population was then expanded, aliquoted, and stored in liquid N2, so that the identical cell population could be used for all subsequent in vivo experiments.
The four circles indicated the identical cells.
Using identical cell lines identified previously as representing the extremes of sensitivity, gene signatures representative of sensitivity to paclitaxel, 5-fluorouracil, adriamycin, and cyclophosphamide were developed.
This assay was performed according to manufacturer's instructions using identical cell numbers (10,000) seeded into 96 well plates.
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