To correct for bleed-through, images of HEK293 cells expressing GCaMP6s (in pcDNA3.1) were acquired upon stimulation with 2 μM ionomycin in varying external CaCl2 concentrations, using the identical acquisition settings as for the acquisition of the calibration curve.
The network templates we used were chosen because they were defined from a large dataset (675 subjects), provided components that clearly represented the four cortical networks of interest for our hypotheses, and were based on data using the identical acquisition protocol as in our study (Schultz et al., 2014).
In a lecture at Leicester University in November 2004, Sir Stephen Sedley called for the identical reform, using the identical argument.
All images were obtained during a single imaging session at 20× magnification, using identical acquisition parameters, including camera exposure time, gain, gamma, offset, and fluorescence illumination intensity.
These results were achieved using identical acquisition and image interpretation parameters.
To explore this, we generated HeLa cell lines stably expressing GFP or GFP ΔN320 GRASP65 by lentiviral transduction and loaded these cell lines with mitotracker red for imaging using identical acquisition parameters.
An identical set of contrast-enhanced T1-weighted images was obtained immediately following the Gd-DTPA administration, using the same acquisition parameters.
Importantly, all patients were scanned using the same scanner and an identical acquisition protocol (including the same orientation and positioning), and all analyses were performed using the same settings.
However, repeated MR scanning of the same subject, even if using the same scanner and acquisition parameters, does not result in identical images due to small changes in image orientation, changes in prescan parameters, and magnetic field instability.
To assess activation specific to acquisition and extinction learning, we used the contrasts acquisition vs. extinction and extinction vs. acquisition.
Seven simulations were performed to replicate typical image data acquired for patients with ET, using identical data acquisition and tomographic reconstruction procedures as was performed for clinical data, with the only exception being that times per projection were adjusted to provide simulated striatal and background counts typical of mean clinical counts (Fig. 2b and c).
