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cDNA was generated using the iScript cDNA Synthesis kit (Bio-Rad) following the supplier's instructions.
RNA quality was assessed using a nanodrop spectrophotometer (Euroclone); cDNA was generated using the iScript cDNA Synthesis kit (BIO-RAD) following the supplier's instructions.
Cellular RNA was used to perform cDNA synthesis using the iScript cDNA synthesis kit (Bio-Rad).
One microgram RNA was converted to cDNA using the iScript cDNA Synthesis kit (BioRad).
cDNA was prepared using the iScript cDNA Synthesis Kit (BioRad USA, Hercules, California).
First-strand cDNA was synthesized using the iSCRIPT cDNA SYNTHESIS KIT from Biorad.
One µg of total RNA was reverse transcribed using the iScript cDNA Synthesis Kit (Biorad, Hercules, CA).
RNA concentrations were determined and 1 µg RNA was used for cDNA synthesis, using the iScript cDNA synthesis kit (BioRad).
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For all samples, cDNA was synthesized using the iScript™ cDNA Synthesis Kit (Biorad, Veenendaal, the Netherlands).
RNA was converted to cDNA using the iScript™ cDNA Synthesis Kit (BioRad, Veenendaal, The Netherlands) according to manufacturer's instructions.
Reverse transcription was performed with 0.5 or 1 mg of total RNA using the iScript™ cDNA Synthesis Kit (Biorad, Germany).
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