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For all samples, cDNA was synthesized using the iScript™ cDNA Synthesis Kit (Biorad, Veenendaal, the Netherlands).
RNA was converted to cDNA using the iScript™ cDNA Synthesis Kit (BioRad, Veenendaal, The Netherlands) according to manufacturer's instructions.
Reverse transcription was performed with 0.5 or 1 mg of total RNA using the iScript™ cDNA Synthesis Kit (Biorad, Germany).
Five hundred ng of total RNA was reverse transcribed using the iScript™ cDNA Synthesis Kit (Bio-Rad, Hercules, CA).
from EBV-transformed lymphoblastoid cells and converted into first-strand cDNA using the iScript™ cDNA Synthesis Kit (Bio-Rad).
Purified total RNA (200 ng) was reverse transcribed using the iScript™ cDNA synthesis kit (BioRad).
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First-strand cDNA was synthesized using the iSCRIPT cDNA SYNTHESIS KIT from Biorad.
RNA concentrations were determined and 1 µg RNA was used for cDNA synthesis, using the iScript cDNA synthesis kit (BioRad).
cDNA was generated using the iScript cDNA Synthesis kit (Bio-Rad) following the supplier's instructions.
RNA quality was assessed using a nanodrop spectrophotometer (Euroclone); cDNA was generated using the iScript cDNA Synthesis kit (BIO-RAD) following the supplier's instructions.
Cellular RNA was used to perform cDNA synthesis using the iScript cDNA synthesis kit (Bio-Rad).
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