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Using the human reference FCGR sequences (NCBI Build 36.1) and SNP information from dbSNP built 126, these probes were designed to be specific for FCGR3B gene, avoiding SNPs in FCGR3A that would make it "look" like FCGR3B and, obviously, in the absence of SNPs at the probe ligation site.
The GC content of each chromosome was calculated using the human reference sequence build 37.
Four different match cut-off values for FACS were evaluated when using the human reference (Supplementary Fig. 3).
The paired-end sequencing reads (70 bp) were mapped using the human reference genome (V 37.2), with a mean coverage of 60×.
We classified SNVs based on the dinucleotide sequence context following Lawrence et al. [ 13], where we recorded the nucleotide preceding an SNV using the human reference genome (hg19).
Since the probes on the array were designed using the human reference genome, we followed the approach described in Hernando-Herraez et al. (2013) to compare humans and chimpanzees.
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We used the human reference genome hg19 [ 57] to determine the correct strand and sequence annotation for each probe.
Briefly, we used the human reference genome to derive the soap libraries for the assessment of the mapping results of the bisulfite treatment.
Instead, researchers have used the human reference genome, despite its evident differences from that of a cancer cell line that has been evolving in the laboratory for several decades.
The results for both the synthetic MetaSim dataset and the experimental metagenome dataset, when using the human mitochondrial reference, showed relatively few unique reads for each method.
To avoid such an error, we have assembled lists of interacting proteins by using the human protein reference database (HPRD) [ 15] for every gene identified by GeneRetriever® from the candidate region.
More suggestions(15)
using the same reference
using the HapMap3 reference
using the black reference
using the current reference
using the human mike
using the dynamic reference
using the human adenovirus
using the human activity
using the human recombinant
using the Dutch reference
using the human microphone
using the average reference
using the human body
using the common reference
using the human breast
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