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The mechanism of SHMT inhibition by pemetrexed was investigated in vitro using the human recombinant protein.
The in vitro MMP-14-mediated cleavage studies using the human recombinant MMP-14 catalytic domain revealed an enhanced cleavage rate that directly correlated with the linearity of the embedded peptide substrate sequence.
P2 is a peripheral membrane protein of the myelin sheath, and our aim is to combine structural and functional information obtained using the human recombinant P2 protein to elucidate the structure-function relationships in this abundant myelin component.
DBA/1 mice were immunized using the human recombinant GPI as reported previously [ 3, 4].
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Using the human ChoKα1 and ChoKβ recombinant proteins, we performed an in vitro ChoK activity inhibition assay using increasing MN58b concentrations, determining the IC50 for each enzyme.
We designed two tetracycline-inducible recombinant adenoviruses using the human telomerase reverse transcriptase (hTERT) promoter for transcriptional apoptogene targeting.
Standard curves were created using the corresponding human recombinant proteins (hRPs).
The BEVS method was successfully used to produce the human recombinant papillomavirus VLP cancer vaccine (Cervarix).
The kit uses the human-derived recombinant PPARγ LBD tagged with an N-terminal GST-tag and a selective fluorescent PPARγ ligand (PPARγ Green).
Using the recombinant human LRRK2 ROC domain isolated from other regions of the protein, we have proposed that Arg stabilizes the interface of a ROC ROC dimer (Deng et al., 2008).
Also, this finding suggests that the detection system is specific for zona pellucida, and that further refinement of the assay system by using pure human recombinant zona pellucida protein might be useful.
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