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(a) Predicted protein protein interactions between PTEN, TP53, PML, DAXX and MCRS1 using the human protein interaction map.
Searches were run using the human protein subdatabase of the NCBI (http://www.ncbi.nlm.nih.gov, June 2003-November 2006).
We illustrate this phenomenon using the human protein GRB2, an epidermal growth factor receptor-binding protein that is known to mediate several cellular signalling cascades (Fig. 1).
Missing exon sequences were obtained from BLAT searches in the dog genome assembly using the human protein sequence as a query.
Genomic loci for neighboring genes were determined as above, using the human protein sequence and tblastn algorithm in either the Zebrafish Genome or in the Clone Database.
To avoid such an error, we have assembled lists of interacting proteins by using the human protein reference database (HPRD) [ 15] for every gene identified by GeneRetriever® from the candidate region.
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We verified this interaction using the human proteins and observed co-precipitation of hSkp1 with His6-tagged hHsp90α only in the presence of hSgt1.
We used the human protein interaction network as the global network to evaluate the prioritizing performance of VS and other two methods.
We used the human protein atlas (http://www.proteinatlas.org/) to confirm expression of twenty eight genes predicted by the microarray data to have elevated mesangial cell expression.
Based on the microarray results of the gene expression omnibus (GEO) database (GSE2435 and GSE31040, starvation-induced autophagy model), we used the human protein reference database (HPRD) to obtain the protein-protein interaction (PPI) network.
In our hands, purified hPros1 was more stable biochemically and often more potent than rmPros1, and we therefore used the human protein for the majority of the Pros1 experiments described below.
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use the human protein
using the human capital
using the human kinome
using the recombinant protein
using the human mike
using the human adenovirus
using the human activity
using the human recombinant
using the human microphone
using the human breast
using the human genome
using the human exome
using the human face
using the human voice
using the human yardstick
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