Here, we used ANIA to study the human kinome, and our predictions indicate that 14-3-3s 14-3-3s 14-3-3sth up to 40% of humay kinteract
In order to better and more extensively characterize the implication of kinases in the AhR cascade, we have analyzed the consequences of kinase expression knock-down on TCDD-mediated up-regulation of CYP1A1 activity, using a siRNA library covering the human kinome.
To increase the confidence in hits identified in the proteomic screen, we intersected a high-throughput, lenti-virally delivered shRNA screen designed against the human kinome, which used an AML differentiation signature readout.
By using synthetic siRNA-mediated RNAi screens of the human kinome, phosphatome, and about 300 additional genes, we identified a subset of 150 genes that, when silenced, enhance TRAIL-induced caspase-3/7 activation in MB231 cells.
It remains unclear whether this approach reflects the true pharmacodynamic properties of Plk1 inhibitors, since these assays often address only small portions of the human kinome and typically use a recombinant kinase and an artificial substrate.
The sequence-based kinase distance matrix was calculated using T-Rex [52] from the tree file obtained from the human kinome project [53].
We used ANIA to map known and candidate 14-3-3-binding 14-3-3-binding 14-3-3-binding 14-3-3-bindingf thenzymes kinome.
Using the DoS-based Dendrogram (based on the DoS residues predicted by KINspect), we have provided an alternative evolutionary explanation of the human kinome, which we argue, more accurately reflects functional diversity and specificity evolution.
To classify the mutations in the human kinome as disease-associated or neutral according to the sequence features of the mutations, we used a Support Vector Machine (SVM).
However, their use in the clinic warrants further studies to establish and eventually improve their selectivity over the human kinome [ 18].
