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Secreted and intracellular light chain was detected using the human kappa or lambda ELISA quantification kit (Bethyl Laboratories) according to the manufacturer's instructions.
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Here, we used a variable heavy-chain (VHH) domain specific for the human kappa light chain to assist in the structure determination of three therapeutic Fabs that were recalcitrant to crystallization on their own.
In this study, homology model of the human kappa opioid receptor was developed by MODELLER using the X-ray crystal structure of bovine rhodopsin as template.
All receptors were based on the human kappa-opioid RASSL called Ro2 [ 12].
Inter- observer agreement was quantified using the kappa statistic, wherein a kappa value of 0.61 to 1.0 indicates substantial agreement.
The algorithm performance was compared with the performance of four human experts by using the Kappa coefficient of agreement.
Agreement among observers was measured using the multirater kappa measure.
Fleiss' kappa was calculated using the online kappa calculator (Randolph 2008).
Concordance between reviewers was assessed using the weighted kappa statistic.
Test-retest reliability was estimated using the weighted kappa statistic.
Agreement between reviewers was measured using kappa analysis and the results were reported using the Cohen kappa index.
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