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The same supernatants were also used to determine GM-CSF concentrations, using the "human GM-CSF ELISA kit" (Pierce, Rockford, IL) according to the manufacturer's instructions.
This analysis also indicated that the homologous mouse GM-CSF gene does not undergo silencing in parallel with the human GM-CSF gene in lines J253 and D184.
The potential myelotoxicity of the combination of 3 nℳ paclitaxel and 1 μℳ CYC3 was compared with that seen with 30 nℳ paclitaxel, using the CFU-GM assay with human BM cells.
The membrane conductance (Gm) was calculated from the resistance and CC of the neuron using the formula " Gm = R−1 * CC−1".
The growth factors used were human GM-CSF (90 ng ml−1), SCF (50 ng ml−1), and Flt-3 ligand (200 ng ml−1) alone, and combinations of growth factors GM-CSF+SCF, GM-CSF+SCF+Flt-3 ligand, and SCF+Flt-3 ligand.
GM-based tracking using the optimization framework is also applied to human tracking across non-overlapping cameras.
The anti-GMs have concentrated their campaigns on using the public to pressure MPs not to go along with the pro-GMs.
The role of GM-CSF was investigated using the collagenase-induced instability model of osteoarthritis.
The in vitro transcription of gM or luciferase was performed using the MAXIscript kit (Ambion Inc).
GM-EIA cutoff values were determined using the OD450/620 value of sample/OD450/620 value of control.
Human and mouse GM-CSF and mouse glyceraldehydes phosphate dehydrogenise (GAPDH) mRNA levels were measured by real time PCR analysis of cDNA primed using olio (dot), as previously described [ 20], using the primer sets listed in Table 1.
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