Affymetrix microarray was performed using the Human Gene 1.0 ST microarray chip (Agilent Technologies, Santa Clara, CA, USA).
Affymetrix microarray analysis was performed using the Human Gene 1.0 ST microarray chip.
Microarray hybridization was performed at the UCSF Genomics Core Laboratories (UCSF/Gladstone, San Francisco, CA, USA) by using the Human Gene 1.0 ST.
Exon positions (coordinates) within each human PDE4 gene were determined using the human gene sequence and conserved splice variant amino termini, facultative (not present in all forms) and constitutive exons (present in all forms) within the two sequence BLAST (bl2seq) function from NCBI http://blast.ncbi.nlm.nih.gov/Blast.cgi and then formatted into an exon file for use in Multi PipMaker.
These analyses were restricted to a subset of 21 species for which sequence coverage included a substantial portion of the β-globin gene cluster and used the human gene cluster as reference (supplementary table S1, Supplementary Material online).
The BioGPS database was used to evaluate tissue-specific gene expression using the Human U133A/GNF1H Gene Atlas dataset, GEO-GSE11333, [ 41, 42] ).
In conclusion, we demonstrated that immortalized porcine fibroblasts were successfully established using the human hTERT gene, and the TALENs enabled biallelic gene disruptions in these immortalized cells.
Annotations were performed using the human Ensembl gene ID numbers, to maximize recognition by the DAVID tool.
Each of these UCNEs was classified as intronic, UCNE within an untranslated region (UTR) or intergenic using the human RefSeq gene annotation.
To investigate the mechanism of drug resistance, a PhD student at the Chinese Academy of Medical Science obtained a 39 kb genomic sequence from a Fugu cosmid using the human MDR gene as a probe.
