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The hydrolyzates were analyzed using the HPLC system to measure the concentrations of the simple sugars released.
LC/TOF-MS was performed using the HPLC system described above and LCT premier XE (Waters Corp).
The filtered sample was analysed using the HPLC system consisting of the Waters Delta 600 with 600 Controller, photodiode array detector (Waters 996).
Moreover, menthol was characterized using the HPLC system (Fig. 2) and identified by comparing its retention time (t R ) and UV spectrum with that of the standard compound.
Acetic acid and butyric acid were analyzed using the HPLC system (Waters 1525) equipped with an organic acid analysis column (Aminex HPX-87H, 300 mm × 7.8 mm) and also described in our previous study [ 23].
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The chain length of the primed GAG was determined by measuring the migration time on two tandem G2000SWXL (Tosoh, 7.8 mm × 30 cm) size exclusion columns using the HPLC Hitachi system with an inline radiodetector or fluorescent detector.
Each of 100 ug samples were prepared and quantified using the HPLC-MS/MS system as reported by Xu et al. (2016).
We detected aloe-emodin, chrysophanol, emodin, and rhein (the active ingredients of rhubarb rhizome) using the HPLC-PDA system.
Chromatograms were recorded for the standard compounds of aristolochic acid, fenfluramine, and N-nitroso-fenfluramine using the HPLC-PDA system according to methods described in the Japanese Pharmacopoeia 16 [ 19].
Samples were analyzed by a high-performance liquid chromatograph (HPLC) method using the Jasco HPLC system (Jasco, Inc., Easton, MD, USA) and a Bio-Rad reagents kit (Bio-Rad Laboratories, Inc., Hercules, CA, USA).
The purity of the product was analysed using the analytical HPLC system, and specific activity (SA) was determined by HPLC based on a calibration curve (performed in triplicate with four concentrations of parent prucalopride in the range of 1.10−4 to 1.10−7 mol L−1) and measurement of the ultraviolet (UV) signal.
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