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The substrate solution was fed at volumetric flow rates of 0.6 mL/h to 30 mL/h using the HPLC pump.
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The Lov amount present in the release buffer was detected using the HPLC method described above.
The HPLC pump system consisted of a WATERS 600 Controller (Waters Corporation) with a 600 Series WATERS Pump (Waters Corporation) and a WATERS 712 WISP Autosampler.
For the measurements described here, de-ionized water (or other liquid sample) was supplied to the central channel of the microfluidic chip using an HPLC pump connected to a pulsation dampener.
n-Heptane (n-C7) with >99%% purity (Sigma-Aldrich) was fed to the catalyst bed using an HPLC pump, and hydrogen of high purity was introduced to give an H2/n-C7 molaratioiofof 9.
While the autosampler and one of the HPLC pumps were used for the SPE cleanup of one sample, the 10-port switching valve, the second HPLC pump, and the mass spectrometer were used to collect data from the previous sample (Ye et al. 2006).
Constant flow was maintained using an HPLC pump.
Chiral chromatography was performed on a LUX-1 cellulose column (3 μm, 150×2.0 mm; Phenomenex) using an HPLC pump (Alliance 2695; Waters) coupled to a triple-quadrupole mass spectrometer with electrospray probe (Quatro Ultima; Waters).
The total flow rate of the HPLC pumps was 229 μL/min and detection took place using UV at 254 nm.
The total flow rate of the HPLC pumps was 162 μL/min and detection took place using a fluorescence detector (λex 279 nm; λem 440 nm).
For SCX chromatography using the LC-20AB HPLC Pump system (Shimadzu), the peptide from digestion was reconstituted with 4 mL buffer A (25 mM NaH2PO4 in 25% ACN, pH2.7) and loaded onto a 4.6 × 250 mm Ultremex SCX column containing 5-μm Phenomenex(Phenomenex, CA, USA).
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