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Hepatic concentrations of the above three and other metabolites were therefore determined in the present experiments, using the HPLC methodology of Badawy and Morgan (2010) under the conditions described in the preceding paper (Badawy et al., 2011).
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In addition, numerous methods have been developed to increase the detection levels (Burriel Marti et al. 2000; Hancock 1984; Kubickova et al. 2011; Ersser and Davey 1991; Davey and Ersser 1990) and many authors continue using the HPLC methodologies to resolve amino acids in multiple mixture analysis (Molnar-Perl 2003; Haynes et al. 1991; Koros et al. 2007; Perry et al. 2009b).
We quantify HOOH using the HPLC-fluorescence method described previously.
Of the A1C values not from the central laboratory, three were obtained at laboratories using the same methodology (HPLC), certified by the National Glycohemoglobin Standardization Program as traceable to the Diabetes Control and Complications Trial reference method, and two were obtained via DCA 2000 immunoassay, which has high correlation with the HPLC method (13).
The purified malleins were characterized by HPLC using the methodology described above.
Gametocytemia was determined using the same methodology.
Complications were identified using the AHRQ Hospital-level PSI methodology.
They are assessed using the standard methodology.
P% was calculated using the same methodology.
To determine whether there were changes in sugar level, we used HPLC methodology to analyze sugar content in leaves of 35-day old Camelina.
Using the methodology presented in Sect.
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