Sentence examples for using the hplc assay from inspiring English sources
The supernatant was transferred and also analyzed using the HPLC assay.
Intracellular concentrations of ara-GTP, F-ara-A triphosphate (F-ara-ATP), and ara-C triphosphate (ara-CTP) were determined by using the HPLC assay method that we previously established [ 13, 14].
To the collected cell pellets were added 250 μL of 3% sulfosalicylic acid solution followed by sonication for 15 min before being transferred to a 1.5 mL Eppendorf tube for protein thiol and total thiol determination using the HPLC assay as described below.
25-hydroxyvitamin D2 was detectable in only 20% (n = 40) of our subjects using the HPLC-APCI-MS assay.
TeCBH1 -TrCBM-C and C.l.CBH2b were purified using chromatography methods for use as protein standards in the HPLC assay (Additional file 5).
The HPLC assay was conducted using a UV detection wavelength of 259 nm.
For the HPLC assay, the lower limit of detection of xylitol, using the standard curve, is 0.2 ng/mL.
The HPLC assay is based on the IFU N° 71, 1998 method, measured at pH 1, 510 nm using cyanidin chloride (Sigma Aldrich) for the reference standard.
The internal standard for the HPLC assay was ertapenem.
Thus, the HPLC assay showed good repeatability under optimized conditions.
These were analysed for Sotalol concentration using a HPLC assay.
