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The color difference values indicated that indigotin dyed silk showed higher color difference in alum mordanted silk than in iron mordanted silk, whereas it was opposite when the change of dye concentration was examined using the HPLC analysis.
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The same plant materials used for quantitative real-time PCR were used for the HPLC analysis of phenylpropanoid accumulation.
The supernatant (hydrolysate liquor) was used in the HPLC analysis to measure the released sugars, and the remaining FP and CL pellets were used in the DNS assay to measure the reducing sugar ends.
The evaporated sample was dissolved in acetonitrile, and a 1-ml volume was used for the HPLC analysis.
The parameters used in the HPLC analysis were as follows: the mobile phase consisted of acetonitrile water (60 40, v/v); the wavelength of UV detector was set at 229 nm; flow rate was set at of 1.0 mL min 1; the injection volume was 20 μL.
Four commercially available immobilized amylose-derived CSPs (Chiralpak IA-3, Chiralpak ID-3, Chiralpak IE-3 and Chiralpak IF-3) were used in the HPLC analysis of the chiral sulfoxides albendazole (ABZ-SO) and fenbendazole (FBZ-SO) and their in vivo sulfide precursor (ABZ and FBZ) and sulfone metabolite (ABZ-SO2 and FBZ-SO2) under organic aqueous mode.
The column used in the HPLC analysis was an ICSep ICE-COREGEL 87H3 column (Transgenomic, USA).
All supernatants were combined together and used for the HPLC analysis of photosynthetic pigments.
Several peaks that did not correspond to the standards used in the HPLC analysis were observed in the chromatogram of the plant extract, between retention times 15 25 min. To evaluate the cytotoxicity of the plant extracts, breast cancer cells (MCF-7) were treated with varying concentrations of the extracts and the MTT assay was performed.
0.1 ml of the supernatant was diluted 10 times and 1 ml of the sample was taken and placed into a numbered HPLC vial for sugar analysis using the HPLC (DIONEX ICS 3000) Reagent-FreeTM Ion Chromatograph equipped with Dionex ICS-3000 systelectrochemicalical detection using ED 40 and computer controller.
After degrading the silk dyeings in 100°C oven up to 7 days, dye was extracted from each silk and the extracts were analyzed using the HPLC-DAD-MS analysis.
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