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In presence of abnormality in the eye (pathological regions or exudates), using the histogram analysis of optic disc is more effective.
Thus, using techniques that measure the damage to the AF, rather than looking at the entire LH, we replicated the results obtained using the histogram analysis.
Using the histogram analysis approach described in the Materials and Methods, significant reductions in Ktrans were seen in both the 20% most-enhancing (rim) and the remaining least-enhancing (core) parts of tumours.
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The picture was analyzed in Photoshop using the Histogram function.
The resulting histograms showed continuous overlap, and the unbiased probability was reconstructed using the Weighted Histogram Analysis Method (WHAM) (34) and the WHAM code from the Grossfield Lab (University of Rochester, Rochester, NY) to calculate the free energy as a function of the distance around the transition states.
The PMF is then constructed along the z direction using the weighted histogram analysis method [44] and the center of mass coordinates.
The potential of mean force was calculated by using the weighted histogram analysis method [ 61].
Gel images were quantified using the Photoshop Histogram Analysis and was plotted using Microsoft excel and is shown as relative expression level after normalized by controls.
To estimate the convergence of the system, we have used the approach of Yang et al. Then, the PMF profile was calculated using the weighted histogram analysis method (WHAM) as implemented by Grossfield (http://membrane.urmc.rochester.edu/content/wham/, version 2.0.9), and errors were estimated using the uncorrelated data (see Supporting Information, text and Figure S1).
From populations of D. parvula and D. retrocurva used for the vertebrate predation experiment, we additionally quantified the relative transparency using a dissecting microscope and the "Histogram" analysis of ImageJ.
For this simulation, various probability distribution functions are used, which are based on the histogram analysis of the collected data.
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