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GUS activity was determined using the histochemical techniques described previously [ 57].
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A previous study of ours using the histochemical technique of autometallographic silver amplification (AMG) found heavy metals in spinal motor neurons in patients with MND and controls, but the LC was not examined in that study [ 3].
The senescence-associated β-galactosidase (SABG) assay was performed using the histochemical staining kit from Sigma-Aldrich [23].
For example, the histochemical techniques and phytochemical methods have been applied in the distribution and accumulation of active components in Sinomenium acutum, Aloe vera var.
We have now used this histochemical technique to measure the somatic mutation frequency in the uninvolved colon of 12 heterozygous patients with HNPCC, 15 with ileocaecal Crohn's disease and 16 with sporadic colorectal cancer (CRC).
We have previously used the mPAS histochemical technique in human colorectal mucosa to identify a polymorphism for O-acetyltransferase activity that shows monogenic inheritance and to show that crypt-restricted loss of O-acetyltransferase activity in heterozygotes is due to somatic mutation.
The enzyme was stained using standard histochemical techniques [ 43].
Although previous work focused on lipid changes detectable in the SN using histochemical techniques [5], we have chosen not to focus our present study on the SN for two reasons.
Using standard histochemical techniques, serial sections were stained with hematoxylin and eosin stains (Chang et al. 2001).
At time of necropsy, tumor tissues were fixed in 10% neutral buffered formalin and paraffin embedded using standard histochemical techniques.
Crown tissues were collected from plants and fixed and embedded in Lambwax (Raymond A. Lamb Ltd., Eastbourne, BN23 6QE, UK) using standard histochemical techniques [ 52].
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