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CT was calculated using the highest sample CT value (i.e., the sample with the lowest target expression) as an arbitrary constant to be subtracted from all other CT sample values.
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Using the high sampling rate of Athabasca data with a low-noise environment, we were able to obtain a closer look at the inner structure of VLF/ELF emissions, including chorus, and follow the variations of the polarization angle for several types of these emissions, including chorus waves, QP emissions, and hiss.
As requested by the software, Ct values were converted to linear expression quantities by 2-ΔCt using the highest expressed sample as calibrator.
In both the spiked and unspiked samples, the concentration of influenza virus sampled using the filter cassette was higher than that using the high-volume sampler.
Our results (Table 2) show that virus concentrations detected using the PTFE cassette were all higher than those detected using the high-volume sampler.
Recent epidemiologic studies have found collection of dust samples using the high-volume surface sampler (HVS3) to be expensive and cumbersome.
Including these patients in the analysis by using the highest HBP value in the two samples increased the AUC to 0.85, suggesting that more frequent testing might further increase the utility of plasma HBP for prediction of severe sepsis.
In WOEAS, settled dust was collected using the High Volume Surface Sampling System (HVS3) vacuum [ 26], while TCHEQ used the Shop-Vac vacuum (Model: QAM70, 7.0 Amps), another high volume device, equipped with Dust Sampling Socks (X-Cell 100, Midwest Filtration, Cincinnati, OH, USA).
Total RNA was extracted from FFPE human gastric tissues (10 non-tumour samples and 15 GAC samples) using the High Pure FFPE RNA miRNA Isolation Kit (Roche Diagnostic, Basel, Switzerland); clinicopathological characteristics of these samples are listed in Table 2.
Each RNA sample was diluted to 10 ng/µl using RNase-free water, and cDNA was synthesized from each sample using the High Capacity cDNA Reverse Transcription Kit (Life Technologies).
We now use the highest coverage samples for our main metagene figures and include the other replicates as supplements to Figures 2 and 3. We use the average of three replicates for codon-specific values.
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