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The GO analysis of BrGRF proteins was carried out using the GO term analysis tool in Gramene (http://www.geneontology.org/) developed by the GO consortium Conserved motifs in the full-length amino acid sequences of GRF proteins from Chinese cabbage, Arabidopsis, and rice were identified using MEME [ 28].
These GO terms were then assigned to major parental terms for biological process using the GO term Classification Counter http://www.animalgenome.org/bioinfo/tools/countgo.org/bioinfo/tools/countgo
We compared the function of the deleted genes on the basis of their Gene Ontology (GO) annotations [27] by using the GO Term Finder from the Saccharomyces genome database [28].
However, a further functional enrichment analysis is performed on the discovered modules by using the GO TERM FINDER tool [ 91].
Defence-related genes were analysed using the GO term 'defence response' (GO 0006952) (Fig. 5 and Supplementary Fig. S4).
Gene Ontology analysis of hits was done using the GO term enrichment tool DAVID [ 67] and data in the GO database release 2010-10-30 [ 62].
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Using the GO terms of biological processes we identified genes regulated by dex which are associated with the most important processes.
Using an E-value cutoff of 1e-10, plant GOSlim annotations [ 34] were transitively annotated using the GO terms from Arabidopsis.
Curated and noncurated models can also be browsed using the GO terms that are associated with individual models.
According to the GO classification, the identified proteins were clustered using the GO terms "molecular function," "cell function," and "localization" [ 27].
Gene ontology (GO) analysis was also performed using the GO terms indentified for banana supercontigs having an E-value of >10−5 in a BLAST search of Arabidopsis genes in the TAIR databases.
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