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By using the genomic sequence of several related genomes, it is possible to infer how conserved a potential binding sites is, and thus to have a higher confidence in the conserved predicted sites versus the non-conserved sites [3].
Using the genomic sequence of CiLV-C, specific primers and probe were designed and synthesized to amplify a 73 nt amplicon from the movement protein (MP) gene.
Moreover, it has been found that budding yeast nucleosome occupancies measured in vivo by deep sequencing are accurately predictable using the genomic sequence only, indicating that the DNA sequence plays a major role in positioning nucleosomes in vivo [23].
In this study, we employed a systematic peptide identification strategy in which spectra derived from one organism were searched against the genome sequences of progressively more genetically distant neighbor organisms to measure the extent to which proteomic information could be obtained about one species when using the genomic sequence of another.
Contigs were sorted using Mauve [ 43] and concatenated using the genomic sequence of C. ulcerans 809 [ 34] as reference.
Amplifying primers were designed using the genomic sequence for each gene [ 9] in conjunction with Primer3 software [ 10].
Similar(47)
We searched for ABREs and CE3s in the promoter regions of protein-coding genes from A. thaliana and O. sativa using the genomic sequences of Arabidopsis (genome release 5.0, January 2004) and rice (release 4.0, January 2006) downloaded from The Institute for Genomic Research TIGRR) [ 26].
Nevertheless, the protein-level homology that exists between related bacteria makes it possible to extract biological information from the proteome of an organism or microbial community by using the genomic sequences of a near neighbor organism.
The probes were further filtered using the genomic sequences of the parental strains S288c and 59A.
Intronic oligonucleotide primer pairs flanking the coding fragments were designed for both the genes using program primer 3 using the genomic sequences of SLC15A1 and GPC5 gene respectively.
To determine the chromosomal locations of contigs that lacked the above anchors, additional PCR markers such as ISBP and SSR were developed using the genomic sequences of the MTP BAC clones and were used for RH mapping.
More suggestions(15)
using the short sequence
using the genomic outfit
using the multiple sequence
using the genomic tRNA
using the different sequence
using the genomic distance
using the genomic primer
using the synthetic sequence
using the genomic relatedness
using the forward sequence
using the genomic segmentation
using the entire sequence
using the above sequence
using the same sequence
using the genomic rescue
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