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Comparison of the control and automatically predicted GIs was carried out using the genomic display software CIRCOS [ 72].
The UCSC and Ensembl genome browsers are online tools that have been used to display different biological datasets, including genomic variants, expressed sequence tags, and functional genomic data with manually curated annotations [ 17, 18].
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The genomic DNA was purified from biomass using the Genomic DNA Purification Kit (Fermentas).
Genomic DNA was prepared using the Invitrogen genomic extraction kit.
Genomic DNA was extracted by using the PureLink Genomic DNA kit to detect the polymorphic HSP72 SNPs rs1061581 and rs6457452.
The exon and intron structures of individual WRKY III genes were determined using the Gene Structure Display Server (GSDS; http://gsds.cbi.pku.edu.cn/) via alignment of the CDS with their corresponding genomic DNA sequences [ 43].
Exon and intron structures of individual PtrWOXs were illustrated using the Gene Structure Display Server (GSDS, http://gsds.cbi.pku.edu.cn/) [ 43] by aligning the cDNA sequences with the corresponding genomic DNA sequences from http://www.phytozome.net.net
Linc-MD1 was displayed using the UCSC genome browser (Fujita et al., 2010 ); genomic locus conservation was evaluated using the Mammal Cons phastCons30wayPlacental (Siepel et al., 2005 ).
Genomic DNA was extracted using the Wizard® Genomic DNA Purification Kit (Promega) according to producer's specifications.
Diagrams of exon-intron structure were obtained using the online Gene Structure Display Server (GSDS: http://gsds.cbi.pku.edu.cn) with coding sequence (CDS) and genomic sequence [ 24].
In addition, gene structure was investigated using the online Gene Structure Display Server (http://gsds.cbi.pku.edu.cn/) based on full-length mRNA alignments with corresponding genomic sequences, whereas introns are gaps between exons consisting entirely of genomic sequence.
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