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In addition, we also investigated whether or not the bovine paralogs, including the pseudogenes, were subject to gene conversion during evolution using the GENECONV program [25].
Thus, to better understand the possible effect of gene conversion on the molecular profiles observed for HLA genes, we tested for the presence of significant conversion fragments within HLA sequence alignments using the GENECONV program [83], [84] (Table 4).
The sequences were analyzed for gene conversion by using the Geneconv program with default parameters [ 41].
The aligned sequences within the multigene families were used to detect conversion events using the GENECONV program version 1.81 [ 37].
These were analysed within the RDP v2.0 platform [ 60] using the GENECONV program [ 61], which identifies strings of silent polymorphisms shared in sequence triplets that exceed chance expectations.
To further establish whether gene conversion has occurred, and identify the boundaries for each species, we made an analysis using the Geneconv program [ 12] (Table 1).
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To determine whether gene conversion played a similar prominent role in the evolution of vertebrate Ugt1 clusters, we used the Geneconv program [ 41] to search for gene conversion events among Ugt1 variable exons.
Aligned allergen gene CDS were used for analysis of gene conversion using the GENECONV software as described in Sawyer (1989).
Each marker was tested for recombination events using the GENECONV [ 49], MaxChi [ 50], and RDP methods [ 51].
We identified potential gene conversion events within each subfamily using the methods implemented in the GeneConv program [ 51] which identifies identical fragments shared between pairs of nucleotide sequences.
The GENECONV program was used with default settings.
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